Organoids are self-assembled 3D stem cell-derived structures that recapitulate the physiology and microanatomy of their original organs. This technology was first described by Sato et al. in 2009 and allowed for more translatable in vitro studies in epithelial cell lines than were previously possible using 2D cancer cell cultures.
After stem cell isolation, hepatic canine organoids start their life cycle as expanding spheroids, and after ~7 days, they turn into budding and differentiating organoids.
The growth of the hepatic spheroids is represented for every day of growth in this image.
Examples of isolated canine organoid lines that achieved a budding stage of development.